商品详情
是否为危控品： 否生产企业：
密度：纯度：储存条件：
商品描述：
R
sigma-aldrich.com
cOmplete, Mini, EDTA-free
Protease Inhibitor Cocktail Tablets
provided in EASYpack
For life science research only.
Not for use in diagnostic procedures.
y Version: 05
Content Version: March 2021
For the complete inhibition of serine and cysteine proteases during extractions from animal and
plant tissues or cells, yeast, and bacteria
Cat. No. 04 693 159 001
30 tablets
for 10 ml each
Store product at +2 to +8°C.y Version: 05
cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Tablets provided in EASYpack
2
sigma-aldrich.com
1.
General Information............................................................................................................................3
1.1.
Contents................................................................................................................................................................................................... 3
1.2.
Storage and Stability ........................................................................................................................................................................... 3
Storage Conditions (Product) .......................................................................................................................................................... 3
Storage Conditions (Working Solution)........................................................................................................................................ 3
1.3.
Additional Equipment and Reagent required ............................................................................................................................ 3
1.4.
Application.............................................................................................................................................................................................. 3
2.
How to Use this Product ....................................................................................................................4
2.1.
Before you Begin .................................................................................................................................................................................. 4
Safety Information ................................................................................................................................................................................ 4
Working Solution................................................................................................................................................................................... 4
Preparation of 7x-concentrated stock solution......................................................................................................................... 4
2.2.
Parameters .............................................................................................................................................................................................. 4
Absorption............................................................................................................................................................................................... 4
Working Concentration....................................................................................................................................................................... 4
3.
Results ...................................................................................................................................................5
4.
Additional Information on this Product ..........................................................................................5
4.1.
Quality Control....................................................................................................................................................................................... 5
5.
Supplementary Information ..............................................................................................................6
5.1.
Conventions............................................................................................................................................................................................ 6
5.2.
Changes to previous version............................................................................................................................................................ 6
5.3.
Ordering Information........................................................................................................................................................................... 6
5.4.
Trademarks.............................................................................................................................................................................................. 7
5.5.
License Disclaimer............................................................................................................................................................................... 7
5.6.
Regulatory Disclaimer......................................................................................................................................................................... 7
5.7.
Safety Data Sheet................................................................................................................................................................................. 7
5.8.
Contact and Support........................................................................................................................................................................... 71. General Information
y Version: 05
cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Tablets provided in EASYpack
sigma-aldrich.com
3
1. General Information
1.1. Contents
Package
Label
Function / Description
Content
1
cOmplete, Mini, EDTA-free,
Protease inhibitor cocktail
tablets
• Individually packaged tablets.
• Each tablet is sufficient for a
volume of 10 ml solution.
30 foil blister packs,
1 tablet each
1.2. Storage and Stability
Storage Conditions (Product)
When stored at +2 to +8°C, the product is stable through the expiration date printed on the label.
Package
Label
Storage
1
cOmplete, Mini, EDTA-free,
Protease inhibitor cocktail tablets
Store dry at +2 to +8°C.
Storage Conditions (Working Solution)
Store stock solution at +2 to +8°C for 1 to 2 weeks or −15 to −25°C for at least 12 weeks.
1.3. Additional Equipment and Reagent required
For preparation of stock solution
• Double-distilled water or 100 mM phosphate buffer, pH 7.0
For extraction in the acid pH range
• Pepstatin*
For protection of phosphatases
• PhosSTOP, Phosphatase inhibitor cocktail tablets in EASYpacks*
For determination of proteolytic activity
• Universal Protease Substrate, Casein, resorufin-labeled*
1.4. Application
cOmplete, Mini, EDTA-free, Protease inhibitor cocktail tablets are used for the inhibition of a broad spectrum of serine
and cysteine proteases. The inhibitor is ideal for the inhibition of proteolytic activity in smaller volumes, in which EDTA
may interfere with protein stability or subsequent assays or purification methods, such as IMAC (Immobilized Metal
Affinity Chromatography). Due to the optimized composition of the tablets, they show excellent inhibition effects and
are well suited for the protection of proteins isolated from:
• Mammalian tissue
• Bacteria
• Yeast
• Plants
cOmplete, Mini, EDTA-free contains both irreversible and reversible protease inhibitors. In contrast to other cOmplete
tablets, they do not contain EDTA, leaving the stability and the function of metal-dependent proteins unaffected. The
affinity purification of Poly-His tagged fusion proteins via IMAC is also facilitated (no dialysis necessary).
Metalloproteases and aspartic proteases are not inhibited.2. How to Use this Product
y Version: 05
cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Tablets provided in EASYpack
4
sigma-aldrich.com
2. How to Use this Product
2.1. Before you Begin
Safety Information
Do not eat the tablets.
Working Solution
Carefully push the tablet through the foil packaging using the base of your thumb (not fingernail) to
prevent the breakage of tablets.
One cOmplete, Mini, EDTA-free Protease inhibitor tablet is sufficient for the inhibition of the proteolytic activity in
10 ml extraction solution. If very high proteolytic activity is present, use one tablet for 7 ml extraction buffer. Tablets
can be added directly to the extraction medium. Alternatively, a 7x-concentrated stock solution can be prepared.
If it is necessary to inhibit proteolytic activity in a larger volume, use cOmplete, EDTA-free* (1 tablet for 50 ml
extraction solution). The composition of the cOmplete tablet is identical to the normal cOmplete, Mini, EDTA-free
tablet, therefore comparable results are achieved with both products.
Preparation of 7x-concentrated stock solution
Dissolve one cOmplete, Mini, EDTA-free Protease inhibitor tablet in 1.5 ml double-distilled water or in 1.5 ml 100 mM
phosphate buffer, pH 7.0.
2.2. Parameters
Absorption
A solution of 1 tablet cOmplete, Mini, EDTA-free inhibitor dissolved in 10 ml water has an absorption of 0.08 at
280 nm.
Working Concentration
1 tablet per 10 ml extraction solution. 3. Results
y Version: 05
cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Tablets provided in EASYpack
sigma-aldrich.com
5
3. Results
cOmplete, Mini, EDTA-free tablets are applied to stabilize those extracts where the stability or activity of metal
containing proteins should not be affected. Since EDTA interferes with IMAC, cOmplete, EDTA-free is preferentially
used in the isolation process of Poly-His tagged fusion proteins. cOmplete, EDTA-free tablets inhibit efficiently serine
and cysteine proteases in a broad range but not metalloproteases.
Occasionally, aspartic proteases (acid proteases) can interfere in isolations from animal tissues or bacterial extracts.
These proteases, however, exhibit pronounced activities only at low pH values. If extraction or single isolation steps
must be performed at this pH range, add Pepstatin* to inhibit aspartic protease activity as a precaution.
To protect proteins from both proteases and phosphatases, combine cOmplete Protease inhibitor cocktail tablets with
PhosSTOP Phosphatase inhibitor cocktail tablets*.
Typical values for the inhibition of different proteases and protease mixtures by cOmplete, Mini, EDTA-free are shown
in the following table. One cOmplete, Mini, EDTA-free tablet was added per 10 ml incubation solution. Proteolytic
activity was determined with the Universal Protease Substrate, Casein, resorufin-labeled*. When extractions or single
step isolations are necessary in the acid pH range, include Pepstatin* along with cOmplete, Mini, EDTA-free tablets to
ensure aspartic (acid) protease inhibition. All experiments were performed at +15 to +25°C.
Protease
respective
protease mixture
Enzyme
concentration
[mg/ml]
pH value
% Inhibition after
immediate addition
to the protease
% Inhibition after
1 hour incubation
with the protease
Pancreas extract
0.02
7.8
84
94
Thermolysin
(Metalloprotease)
0.0005
7.8
7
10
Chymotrypsin
0.002
7.8
90
100
0.02
7.8
60
98
Trypsin
0.02
7.8
70
74
0.002
7.8
81
86
Papain
0.33
7.8
74
87
4. Additional Information on this Product
4.1. Quality Control
The inhibitory activity of each lot is tested with a concentrated pancreas extract. Thereby, the proteolytic activities are
typically inhibited by 94% after one hour (detection with Universal Protease Substrate Casein, resorufin-labeled*). 5. Supplementary Information
y Version: 05
cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Tablets provided in EASYpack
6
sigma-aldrich.com
5. Supplementary Information
5.1. Conventions
To make information consistent and easier to read, the following text conventions and symbols
are used in this document to highlight important information:
Text convention and symbols
Information Note: Additional information about the current topic or procedure.
Important Note: Information critical to the success of the current procedure or use of the product.
etc.
etc.
Stages in a process that usually occur in the order listed.
Steps in a procedure that must be performed in the order listed.
* (Asterisk)
The Asterisk denotes a product available from Roche Diagnostics.
5.2. Changes to previous version
Layout changes.
Editorial changes.
5.3. Ordering Information
Product
Pack Size
Cat. No.
Reagents, kits
PhosSTOP
10 tablets, for 10 ml each
04 906 845 001
20 tablets, for 10 ml each
04 906 837 001
Universal Protease Substrate
40 mg
11 734 334 001
cOmplete, EDTA-free
20 tablets in a glass vial, for 50 ml each
11 873 580 001
3 x 20 tablets in glass vials, for 50 ml each
05 056 489 001
Pepstatin
2 mg
10 253 286 001
10 mg
11 359 053 001
50 mg
11 524 488 001
1 2 3
1 2 3
0321.04728688001
5
Roche Diagnostics GmbH
Sandhofer Strasse 116
68305 Mannheim
Germany
5. Supplementary Information
5.4. Trademarks
PHOSSTOP is a trademark of Roche.
All other product names and trademarks are the property of their respective owners.
5.5. License Disclaimer
For patent license limitations for individual products please refer to:
List of biochemical reagent products.
5.6. Regulatory Disclaimer
For life science research only. Not for use in diagnostic procedures.
5.7. Safety Data Sheet
Please follow the instructions in the Safety Data Sheet (SDS).
5.8. Contact and Support
To ask questions, solve problems, suggest enhancements or report new applications,
please visit our Online Technical Support Site.
To call, write, fax, or email us, visit sigma-aldrich.com, and select your home country. Country-specific contact
information will be displayed.
0321.04728688001
5
